diff --git a/bitbake/lib/bb/cooker.py b/bitbake/lib/bb/cooker.py
index 0008c2fde1..b4851e13ed 100644
--- a/bitbake/lib/bb/cooker.py
+++ b/bitbake/lib/bb/cooker.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 #
 # Copyright (C) 2003, 2004  Chris Larson
 # Copyright (C) 2003, 2004  Phil Blundell
diff --git a/bitbake/lib/bb/cookerdata.py b/bitbake/lib/bb/cookerdata.py
index 842275d530..e64c6c5271 100644
--- a/bitbake/lib/bb/cookerdata.py
+++ b/bitbake/lib/bb/cookerdata.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 #
 # Copyright (C) 2003, 2004  Chris Larson
 # Copyright (C) 2003, 2004  Phil Blundell
diff --git a/bitbake/lib/bb/main.py b/bitbake/lib/bb/main.py
index ca59eb9af8..bd11619966 100755
--- a/bitbake/lib/bb/main.py
+++ b/bitbake/lib/bb/main.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 #
 # Copyright (C) 2003, 2004  Chris Larson
 # Copyright (C) 2003, 2004  Phil Blundell
diff --git a/bitbake/lib/bb/monitordisk.py b/bitbake/lib/bb/monitordisk.py
index 69b25c7720..1a25b0041f 100644
--- a/bitbake/lib/bb/monitordisk.py
+++ b/bitbake/lib/bb/monitordisk.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 #
 # Copyright (C) 2012 Robert Yang
 #
diff --git a/bitbake/lib/bb/namedtuple_with_abc.py b/bitbake/lib/bb/namedtuple_with_abc.py
index c8e1d55c15..646aed6ffd 100644
--- a/bitbake/lib/bb/namedtuple_with_abc.py
+++ b/bitbake/lib/bb/namedtuple_with_abc.py
@@ -1,5 +1,4 @@
 # http://code.activestate.com/recipes/577629-namedtupleabc-abstract-base-class-mix-in-for-named/
-#!/usr/bin/env python
 # Copyright (c) 2011 Jan Kaliszewski (zuo). Available under the MIT License.
 #
 # SPDX-License-Identifier: MIT
diff --git a/bitbake/lib/bb/parse/parse_py/BBHandler.py b/bitbake/lib/bb/parse/parse_py/BBHandler.py
index 889f230f79..6f7cf82b25 100644
--- a/bitbake/lib/bb/parse/parse_py/BBHandler.py
+++ b/bitbake/lib/bb/parse/parse_py/BBHandler.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 """
    class for handling .bb files
 
diff --git a/bitbake/lib/bb/parse/parse_py/ConfHandler.py b/bitbake/lib/bb/parse/parse_py/ConfHandler.py
index 71bf61b761..2e84b913d8 100644
--- a/bitbake/lib/bb/parse/parse_py/ConfHandler.py
+++ b/bitbake/lib/bb/parse/parse_py/ConfHandler.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 """
    class for handling configuration data files
 
diff --git a/bitbake/lib/bb/parse/parse_py/__init__.py b/bitbake/lib/bb/parse/parse_py/__init__.py
index cdebe440dd..f508afa14e 100644
--- a/bitbake/lib/bb/parse/parse_py/__init__.py
+++ b/bitbake/lib/bb/parse/parse_py/__init__.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 """
 BitBake Parsers
 
diff --git a/bitbake/lib/bb/runqueue.py b/bitbake/lib/bb/runqueue.py
index 010b085010..58707db309 100644
--- a/bitbake/lib/bb/runqueue.py
+++ b/bitbake/lib/bb/runqueue.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 """
 BitBake 'RunQueue' implementation
 
diff --git a/bitbake/lib/bb/taskdata.py b/bitbake/lib/bb/taskdata.py
index d13bd7c3bf..8c25e09e8a 100644
--- a/bitbake/lib/bb/taskdata.py
+++ b/bitbake/lib/bb/taskdata.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 """
 BitBake 'TaskData' implementation
 
diff --git a/bitbake/lib/pyinotify.py b/bitbake/lib/pyinotify.py
index 9dcfd0d2ee..1528a22e8d 100644
--- a/bitbake/lib/pyinotify.py
+++ b/bitbake/lib/pyinotify.py
@@ -1,5 +1,4 @@
-#!/usr/bin/env python
-
+#
 # pyinotify.py - python interface to inotify
 # Copyright (c) 2005-2015 Sebastien Martini <seb@dbzteam.org>
 #